.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.49.3. .TH FML-ASM "1" "January 2023" "fml-asm 0.1" "User Commands" .SH NAME fml-asm \- assemble Illumina short reads in small regions .SH SYNOPSIS .B fml-asm [\fI\,options\/\fR] \fI\,\/\fR .SH OPTIONS .TP \fB\-e\fR INT k\-mer length for error correction (0 for auto; \fB\-1\fR to disable) [0] .TP \fB\-c\fR INT1[,INT2] range of k\-mer & read count thresholds for ec and graph cleaning [4,8] .TP \fB\-l\fR INT min overlap length during initial assembly [33] .TP \fB\-r\fR FLOAT drop an overlap if its length is below maxOvlpLen*FLOAT [0.7] .TP \fB\-t\fR INT number of threads (don't use multi\-threading for small data sets) [1] .TP \fB\-d\fR INT retain a bubble if one side is longer than the other side by >INT\-bp [50] .TP \fB\-A\fR discard heterozygotes (apply this to assemble bacterial genomes; override \fB\-O\fR) .TP \fB\-O\fR don't apply aggressive tip trimming .TP \fB\-g\fR output the assembly graph in the GFA format