.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.49.3. .TH MAC3_BDGOPT "1" "February 2024" "macs3 bdgopt 3.0.1" "User Commands" .SH NAME mac3_bdgopt \- Model\-based Analysis for ChIP\-Sequencing .SH DESCRIPTION usage: macs3 bdgopt [\-h] \fB\-i\fR IFILE [\-m {multiply,add,p2q,max,min}] .TP [\-p [EXTRAPARAM ...]] [\-\-outdir OUTDIR] \fB\-o\fR OFILE [\-\-verbose VERBOSE] .SS "options:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-i\fR IFILE, \fB\-\-ifile\fR IFILE MACS score in bedGraph. Note: this must be a bedGraph file covering the ENTIRE genome. REQUIRED .TP \fB\-m\fR {multiply,add,p2q,max,min}, \fB\-\-method\fR {multiply,add,p2q,max,min} Method to modify the score column of bedGraph file. Available choices are: multiply, add, max, min, or p2q. 1) multiply, the EXTRAPARAM is required and will be multiplied to the score column. If you intend to divide the score column by X, use value of 1/X as EXTRAPARAM. 2) add, the EXTRAPARAM is required and will be added to the score column. If you intend to subtract the score column by X, use value of \fB\-X\fR as EXTRAPARAM. 3) max, the EXTRAPARAM is required and will take the maximum value between score and the EXTRAPARAM. 4) min, the EXTRAPARAM is required and will take the minimum value between score and the EXTRAPARAM. 5) p2q, this will convert p\-value scores to q\-value scores using Benjamini\-Hochberg process. The EXTRAPARAM is not required. This method assumes the scores are \fB\-log10\fR p\-value from MACS3. Any other types of score will cause unexpected errors. .TP \fB\-p\fR [EXTRAPARAM ...], \fB\-\-extra\-param\fR [EXTRAPARAM ...] The extra parameter for METHOD. Check the detail of \fB\-m\fR option. .TP \fB\-\-outdir\fR OUTDIR If specified all output files will be written to that directory. Default: the current working directory .TP \fB\-o\fR OFILE, \fB\-\-ofile\fR OFILE Output BEDGraph filename. .TP \fB\-\-verbose\fR VERBOSE Set verbose level of runtime message. 0: only show critical message, 1: show additional warning message, 2: show process information, 3: show debug messages. DEFAULT:2