NAME¶
domainnr - Removes redundant domains from a DCF file.
SYNOPSIS¶
domainnr -dcfinfile infile
[-datafile matrixf]
-retain toggle
-node list
-mode list
-threshold float
-threshlow float
-threshup float
[-gapopen float]
[-gapextend float]
-dcfoutfile outfile
-redoutfile outfile
-logfile outfile
domainnr -help
DESCRIPTION¶
domainnr is a command line program from EMBOSS (“the
European Molecular Biology Open Software Suite”). It is part of the
"Utils:Database creation" command group(s).
OPTIONS¶
-dcfinfile infile
This option specifies name of DCF file (domain
classification file) (input). A 'domain classification file' contains
classification and other data for domains from SCOP or CATH, in DCF format
(EMBL-like). The files are generated by using SCOPPARSE and CATHPARSE. Domain
sequence information can be added to the file by using DOMAINSEQS.
-datafile matrixf
This option specifies the residue substitution matrix.
This is used for sequence comparison. Default value: EBLOSUM62
-retain toggle
This option specifies whether to write redundant domains
to a separate file. If this option is selected, redundant domains are written
to a separate output file. Default value: N
Required section¶
-node list
This option specifies the node for redundancy removal.
Redundancy can be removed at any specified node in the SCOP or CATH
hierarchies. For example by selecting 'Class' entries belonging to the same
Class will be non-redundant. Default value: 1
-mode list
This option specifies whether to remove redundancy at a
single threshold % sequence similarity or remove redundancy outside a range of
acceptable threshold % similarity. All permutations of pair-wise sequence
alignments are calculated for each domain family in turn using the EMBOSS
implementation of the Needleman and Wunsch global alignment algorithm.
Redundant sequences are removed in one of two modes as follows: (i) If a pair
of proteins achieve greater than a threshold percentage sequence similarity
(specified by the user) the shortest sequence is discarded. (ii) If a pair of
proteins have a percentage sequence similarity that lies outside an acceptable
range (specified by the user) the shortest sequence is discarded. Default
value: 1
-threshold float
This option specifies the % sequence identity redundancy
threshold, which determines the redundancy calculation. If a pair of proteins
achieve greater than this threshold the shortest sequence is discarded.
Default value: 95.0
-threshlow float
This option specifies the % sequence identity redundancy
threshold, which determines the redundancy calculation. If a pair of proteins
have a percentage sequence similarity that lies outside an acceptable range
the shortest sequence is discarded. Default value: 30.0
-threshup float
This option specifies the % sequence identity redundancy
threshold, which determines the redundancy calculation. If a pair of proteins
have a percentage sequence similarity that lies outside an acceptable range
the shortest sequence is discarded. Default value: 90.0
Additional section¶
-gapopen float
This option specifies the gap insertion penalty. This is
the score taken away when a gap is created. The best value depends on the
choice of comparison matrix. The default value assumes you are using the
EBLOSUM62 matrix for protein sequences, and the EDNAFULL matrix for nucleotide
sequences. Default value: 10
-gapextend float
This option specifies the gap extension penalty. This is
added to the standard gap penalty for each base or residue in the gap. This is
how long gaps are penalized. Usually you will expect a few long gaps rather
than many short gaps, so the gap extension penalty should be lower than the
gap penalty. Default value: 0.5
Output section¶
-dcfoutfile outfile
This option specifies the name of non-redundant DCF file
(domain classification file) (output). A 'domain classification file' contains
classification and other data for domains from SCOP or CATH, in DCF format
(EMBL-like). The files are generated by using SCOPPARSE and CATHPARSE. Domain
sequence information can be added to the file by using DOMAINSEQS. Default
value: test.scop
-redoutfile outfile
This option specifies the name of DCF file (domain
classification file) for redundant sequences (output). A 'domain
classification file' contains classification and other data for domains from
SCOP or CATH, in DCF format (EMBL-like). The files are generated by using
SCOPPARSE and CATHPARSE. Domain sequence information can be added to the file
by using DOMAINSEQS.
-logfile outfile
This option specifies the name of log file for the build.
The log file contains messages about any errors arising while domainnr ran.
Default value: domainnr.log
SEE ALSO¶
domainnr is fully documented via the tfm(1) system.
AUTHOR¶
Debian Med Packaging Team
<debian-med-packaging@lists.alioth.debian.org>
Wrote the script used to autogenerate this manual
page.
COPYRIGHT¶
This manual page was autogenerated from an Ajax Control Definition
of the EMBOSS package. It can be redistributed under the same terms as
EMBOSS itself.
