NAME¶

Bio::Restriction::Enzyme::MultiCut - A single restriction endonuclease

SYNOPSIS¶

  # set up a single restriction enzyme. This contains lots of
  # information about the enzyme that is generally parsed from a
  # rebase file and can then be read back
  use Bio::Restriction::Enzyme;

DESCRIPTION¶

This module defines a restriction endonuclease class where one object represents one of the distinct recognition sites for that enzyme. The method others stores references to other objects with alternative sites.

In this schema each object within an EnzymeCollection can be checked for matching a sequence.

REBASE report notation "Bsp24I (8/13)GACNNNNNNTGG(12/7)" means:

   Bsp24I
             5'      ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^   3'
             3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^        5'

FEEDBACK¶

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

  https://github.com/bioperl/bioperl-live/issues

AUTHOR¶

Heikki Lehvaslaiho, heikki-at-bioperl-dot-org

CONTRIBUTORS¶

Rob Edwards, redwards@utmem.edu

COPYRIGHT¶

Copyright (c) 2003 Rob Edwards.

Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights Reserved. This module is free software; you can redistribute it and/or modify it under the same terms as Perl itself.

SEE ALSO¶

Bio::Restriction::Enzyme, Bio::Restriction::Analysis, Bio::Restriction::EnzymeCollection

APPENDIX¶

Methods beginning with a leading underscore are considered private and are intended for internal use by this module. They are not considered part of the public interface and are described here for documentation purposes only.

 Title     : new
 Function
 Function  : Initializes the enzyme object
 Returns   : The Restriction::Enzyme::MultiCut object
 Argument  :

 Title     : others
 Usage     : $re->others(@enz_obj_array);
 Function  : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
 Arguments : optional array of Enzyme::MultiCut objects
 Returns   : array of Enzyme objects

 Title     : purge_others
 Usage     : $re->purge_references();
 Function  : Purges the set of references for this enzyme
 Arguments : 
 Returns   :