'\" t .\" Title: reapr-break .\" Author: [see the "AUTHOR(S)" section] .\" Generator: Asciidoctor 2.0.20 .\" Date: .\" Manual: \ \& .\" Source: \ \& .\" Language: English .\" .TH "REAPR\-BREAK" "1" "" "\ \&" "\ \&" .ie \n(.g .ds Aq \(aq .el .ds Aq ' .ss \n[.ss] 0 .nh .ad l .de URL \fI\\$2\fP <\\$1>\\$3 .. .als MTO URL .if \n[.g] \{\ . mso www.tmac . am URL . ad l . . . am MTO . ad l . . . LINKSTYLE blue R < > .\} .SH "NAME" reapr-break \- make broken assembly via REAPR .SH "SYNOPSIS" .sp \fBreapr break\fP [options] .SH "DESCRIPTION" .sp is the errors GFF file made when running \fBscore\fP. .SH "OPTIONS" .sp \fB\-a\fP .RS 4 Aggressive breaking: break contigs at any FCD or low_frag error, as opposed to the default of replacing with Ns. Contigs are broken at the midpoint of each error. Also see option \fB\-t\fP. Incompatible with \fB\-b\fP. .RE .sp \fB\-b\fP .RS 4 Ignore FCD and low fragment coverage errors that do not contain a gap (the default is to replace these with Ns). Incompatible with \fB\-a\fP. .RE .sp \fB\-e\fP .RS 4 Minimum FCD error [0] .RE .sp \fB\-l\fP .RS 4 Minimum sequence length to output [100] .RE .sp \fB\-m\fP .RS 4 Max sequence length to write to the bin. Sequences longer than this are written to the main assembly output. This is to stop long stretches of sequence being lost [999] .RE .sp \fB\-t\fP .RS 4 When \fB\-a\fP is used, use this option to specify how many bases are trimmed off the end of each new contig around a break. \fB\-t\fP N means that, at an FCD error, a contig is broken at the middle coordinate of the error, then N bases are trimmed off each new contig end [0] .RE .SH "SEE ALSO" .sp reapr(1)