table of contents
Bio::Matrix::PSM::ProtPsm(3pm) | User Contributed Perl Documentation | Bio::Matrix::PSM::ProtPsm(3pm) |
NAME¶
Bio::Matrix::PSM::ProtPsm - handle combination of site matricies
SYNOPSIS¶
use Bio::Matrix::PSM::IO; #To get a ProtPsm object from a file use the Psm parser: my $psmIO = Bio::Matrix::PSM::IO->new(-format=>'psiblast', -file=>$file); # Now go through all entities in the file with next_psm, which # returns a Psm object see Bio::Matrix::PSM::IO for detailed # documentation (matrix predictions or matrix sequence matches or # both): while (my $psm=$psmIO->next_psm) { my %psm_header = $psm->header; my $ic = $psm_header{IC}; my $sites = $psm_header{sites}; my $width = $psm_header{width}; my $score = $psm_header{e_val}; my $IUPAC = $psm->IUPAC; my $instances = $psm->instances; foreach my $instance (@{$instances}) { my $id = $instance->primary_id; #Do something with the id } }
DESCRIPTION¶
To handle a combination of site matrices and/or their corresponding sequence matches (instances). This object inherits from Bio::Matrix::PSM::ProtMatrix, so you can methods from that class. It may hold also an array of Bio::Matrix::PSM::InstanceSite object, but you will have to retrieve these through Bio::Matrix::PSM::ProtPsm->instances method (see below). To some extent this is an expanded ProtMatrix object, holding data from analysis that also deal with sequence matches of a particular matrix.
DESIGN ISSUES¶
This does not make too much sense to me I am mixing PSM with PSM sequence matches Though they are very closely related, I am not satisfied by the way this is implemented here. Heikki suggested different objects when one has something like meme But does this mean we have to write a different objects for mast, meme, transfac, theiresias, etc.? To me the best way is to return SiteMatrix object + arrray of InstanceSite objects and then mast will return undef for SiteMatrix and transfac will return undef for InstanceSite. Probably I cannot see some other design issues that might arise from such approach, but it seems more straightforward. Hilmar does not like this because it is an exception from the general BioPerl rules. Should I leave this as an option? Also the header rightfully belongs the driver object, and could be retrieved as hashes. I do not think it can be done any other way, unless we want to create even one more object with very unclear content.
FEEDBACK¶
Mailing Lists¶
User feedback is an integral part of the evolution of this and
other Bioperl modules. Send your comments and suggestions preferably
to one of the Bioperl mailing lists. Your participation is much
appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support¶
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs¶
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
AUTHOR - James Thompson¶
Email tex@biosysadmin.com
DISCLAIMER¶
This software is provided "as is" without warranty of any kind.
SEE ALSO¶
ProtMatrix, meme, transfac, psiblast, InstanceSite
APPENDIX¶
new¶
Title : new Usage : my $psm = Bio::Matrix::PSM::ProtPsm->new( -pS => [ '0', '33', '0', '16', '1', '12', '11', '25' ], -pF => [ '0', '0', '2', '0', '3', '0', '0', '0' ], -pT => [ '0', '8', '7', '10', '1', '2', '7', '8' ], -pN => [ '0', '0', '2', '13', '0', '36', '1', '4' ], -pK => [ '0', '5', '0', '13', '1', '15', '0', '2' ], -pY => [ '0', '0', '0', '0', '0', '0', '0', '0' ], -pE => [ '0', '41', '1', '12', '0', '0', '0', '15' ], -pV => [ '0', '3', '9', '0', '2', '0', '3', '1' ], -pQ => [ '0', '0', '0', '15', '0', '4', '0', '3' ], -pM => [ '100', '0', '66', '0', '2', '0', '0', '0' ], -pC => [ '0', '0', '0', '0', '0', '0', '0', '0' ], -pL => [ '0', '0', '8', '0', '25', '0', '4', '0' ], -pA => [ '0', '10', '1', '9', '2', '0', '22', '16' ], -pW => [ '0', '0', '0', '0', '0', '0', '0', '0' ], -pP => [ '0', '0', '0', '0', '3', '1', '45', '0' ], -pH => [ '0', '0', '0', '0', '0', '0', '1', '0' ], -pD => [ '0', '0', '1', '7', '2', '2', '0', '22' ], -pR => [ '0', '0', '0', '3', '0', '27', '0', '0' ], -pI => [ '0', '0', '3', '0', '59', '1', '2', '3' ], -pG => [ '0', '0', '0', '1', '0', '0', '4', '1' ], -IC => $ic, -sites => $istes, -width => $width, -e_val => $e_val, -instances => $instances, } Function: Creates a new Bio::Matrix::PSM::ProtPsm object Throws : Example : Returns : Bio::Matrix::PSM::Psm object Args : hash
instances¶
Title : instances Usage : my @instances=@{$psm->instances}; Function: Gets/sets the instances (Bio::Matrix::PSM::InstanceSite objects) associated with the Psm object Throws : Example : Returns : array reference (Bio::Matrix::PSM::InstanceSite objects) Args : array reference (Bio::Matrix::PSM::InstanceSite objects)
header¶
Title : header Usage : my %header=$psm->header; my $ic=$psm->header('IC'); Function: Gets the general information, common for most files, dealing with PSM such as information content (IC), score (e-value, etc.), number of sites (sites) and width. This list may expand. The current list should be in @Bio::Matrix::PSM::Psm::HEADER. Returns an epty list if an argument is supplied that is not in @Bio::Matrix::PSM::meme::HEADER. Throws : Example : Returns : hash or string Args : string (IC, e_val...)
matrix¶
Title : matrix Usage : my $matrix = $psm->matrix; Function: Gets/sets the SiteMatrix related information Throws : Example : Returns : Bio::Matrix::PSM::SiteMatrix objects Args : Bio::Matrix::PSM::SiteMatrix objects
2021-08-15 | perl v5.32.1 |