NAME¶

SYNOPSIS¶

DESCRIPTION¶

Extracts subalignments from a Mercator multiple alignment for the features in the GFF file.

OPTIONS¶

-h, --help

show this message

-v, --version

show version information

-t, --type <string>

only look at features of particular type

-D, --data <directory>

path to map, genome and alignment files

-M, --map <directory>

path to map and genome files

-A, --align <directory>

path to alignment files

-L, --lazy

warn, rather than die, if the subalignment can't be obtained

-U, --truncate

truncate unmappable sequence (rather than skipping) and show truncated subalignment

-s, --stockholm

use and display Stockholm-format alignments with conservation statistics (default is multi-FASTA)

-e, --verbose

report progress

PLEASE NOTE: While this program is reasonably fast if the GFF is properly ordered by chromosome and the start and end coordinates of features, it will be *very slow* if the GFF is not sorted. Assumes that the "seqid" or "name" field (the first field) of the GFF entries holds the chromosome name.

Note that the GFF specification defines coordinates to be 1-based and fully-closed, therefore representing the interval [start, end]. Conformance to this specification is assumed internally.

If requested, unmappable sequence will be truncated to the mappable portion; note that the truncation will favor the beginning of the requested sequence.

If a GFF feature is on the - strand, then the corresponding subalignment will be reverse-complemented.

AUTHOR¶