Scroll to navigation

UNICYCLER_SCRUB(1) User Commands UNICYCLER_SCRUB(1)

NAME

unicycler_scrub - read trimming, chimera detection and misassembly detection

SYNOPSIS

unicycler_scrub [-h] -i INPUT -o OUT [-r READS] [--trim TRIM] [--split SPLIT] [--min_split_size MIN_SPLIT_SIZE] [--discard_chimeras] [-t THREADS] [--keep_paf] [--parameters PARAMETERS] [--verbosity VERBOSITY]

DESCRIPTION

Unicycler-scrub - read trimming, chimera detection and misassembly detection

OPTIONS

-h, --help
show this help message and exit
-i INPUT, --input INPUT
These are the reads or assembly to be scrubbed (can be FASTA or FASTQ format
-o OUT, --out OUT
The scrubbed reads or assembly will be saved to this file (will have the same format as the --input file format) or use "none" to not produce an output file
-r READS, --reads READS
These are the reads used to scrub --input (can be FASTA or FASTQ format) (default: same file as --input)
--trim TRIM
The aggressiveness with which the input will be trimmed (0 to 100, where 0 is no trimming and 100 is very aggressive trimming) (default: 50)
--split SPLIT
The aggressiveness with which the input will be split (0 to 100, where 0 is no splitting and 100 is very aggressive splitting) (default: 50)
--min_split_size MIN_SPLIT_SIZE
Parts of split sequences will only be outputted if they are at least this big (default: 1000)
--discard_chimeras
If used, chimeric sequences will be discarded instead of split (default: False)
-t THREADS, --threads THREADS
Number of threads used (default: 4)
--keep_paf
Save the alignments to file (makes repeated runs faster because alignments can be loaded from file) (default: False)
--parameters PARAMETERS
Low-level parameters (for debugging use only) (default: )
--verbosity VERBOSITY
Level of stdout information (default: 1)
0 = no stdout,
1 = basic progress indicators,
2 = extra info,
3 = debugging info

SEE ALSO

unicycler(1)

AUTHOR

This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.
October 2018 unicycler_scrub 0.4.7