NAME¶

fsa_assemble - Constructs contigs from filtered overlaps and corrected reads

SYNOPSIS¶

fsa_assemble [options] filterd_overlaps

DESCRIPTION¶

Constructs contigs from filtered overlaps and corrected reads.

OPTIONS¶

--min_length=INT

minimum length of reads default: 0

--min_identity=DOUBLE

minimum identity of overlaps default: 0

--min_aligned_length=INT

minimum aligned length of overlaps default: 0

--min_contig_length=INT

minimum length of contigs default: 500

--read_file=STRING

read filename default: ""

--overlap_file_type="|m4|m4a|paf|ovl"

overlap file format. "" = filename extension, "m4" = M4 format, "paf" = PAF format generated by minimap2, "ovl" = OVL format generated by FALCON. default: ""

--output_directory=STRING

directory for output files default: "."

--select_branch="no|best"

selecting method when encountering branches in the graph, "no" = do not select any branch, "best" = select the most probable branch default: "no"

--thread_size=INT

number of threads default: 1

--dump=INT

for testing, dump intermediate files default: 0

--run_mode=INT

for testing default: 4

--lfc

deprecated, for testing

--remove_chimer

deprecated, remove chimer node

Usage: fsa_assemble [options] filterd_overlaps

filterd_overlaps

input filename

--min_length=INT

minimum length of reads default: 0

--min_identity=DOUBLE

minimum identity of overlaps default: 0

--min_aligned_length=INT

minimum aligned length of overlaps default: 0

--min_contig_length=INT

minimum length of contigs default: 500

--read_file=STRING

read filename default: ""

--overlap_file_type="|m4|m4a|paf|ovl"

overlap file format. "" = filename extension, "m4" = M4 format, "paf" = PAF format generated by minimap2, "ovl" = OVL format generated by FALCON. default: ""

--output_directory=STRING

directory for output files default: "."

--select_branch="no|best"

selecting method when encountering branches in the graph, "no" = do not select any branch, "best" = select the most probable branch default: "no"

--thread_size=INT

number of threads default: 1

--dump=INT

for testing, dump intermediate files default: 0

--run_mode=INT

for testing default: 4

--lfc

deprecated, for testing

--remove_chimer

deprecated, remove chimer node